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Scientific paper – Characterization of Bacterial Lysates

Sep 22, 2020

We are glad to introduce you this publication from the Department of Biotechnlogy Development, Institute of Hygiene, Faculty of Medicine in Uruguay.

The paper entitled “Characterization of Bacterial Lysates by Use of Matrix-Assisted Laser Desorption–Ionization Time of Flight Mass Spectrometry Fingerprinting” presents a new analytical method for bacterial lysates characterization: MALDI-TOF MS (Matrix-Assisted Laser Desorption-Inoization Time of Flight Mass Spectrometry Mass). The technique allows to obtain a unique mass fingerprint for each microorganism, and is thus ideal for the analysis of monovalent but also polyvalent bacterial lysates products.

This reliable and reproducible method is useful to produce protein profiles after cellular extraction and purification.

It is important to note that Polyvalent bacterial lysates obtained by mechanical lysis, PMBL, resulted in a more defined fingerprints, so a more precise characterization as compared to those obtained by chemical alkaline lysis. This result corroborates our precedent findings on PMBL, showing that mechanical lysis allows a better  preservation of the proteins/antigens structure as compared to chemical lysis.

This MALDI-TOF MS method is a cost-effective tool to characterize bacterial lysates.

Discover the full publication : Biochemistry24_Characterization of Bacterial Lysates